A drag queen.
Origin: "Will and Grace"
Don't mess with that mister sister, or you'll end up looking like a Cher barbie doll gone bad.
po asdfghjkl Септембар 25, 2006
Someone of the male species, that wants and or appears to look like a female.A male that is very feminine, also known as a "shemale"
That gay guy looks like mister sister
po Nicki Новембар 10, 2003
a transgendered person
Chet: That girl looks awfully masculine
John: Haha Chet, that isn't a girl, that's a mister sister
Chet: A what?
John: A mister sister. A shemale. A tranny. You gettin' it?
po SHMOSEPH Мај 7, 2013

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