Top Definition
GUI
Graphical User Interface.

A visual display of files, programs, and program directories designed to make it easier for a person to use a computer.
Microsoft Windows has an easy-to use GUI.
po AYB Април 4, 2003

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Photos & Videos
Gui
A mythological creature, often useless and bad at everything it does.
Man, you're such a gui ...
po Nubita Јун 29, 2016

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the way a dyslexic person would spell "guys"
person one: lol u guis crack me up!

jenny and sue: lmao! did he just say guis!?!?
po meadowlark3000 Мај 11, 2009

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GUI
The noise you make when something enters your asshole
Bill: GUI, GUI, GUI, yea, shove that loaf of bread in my asshole more Steve
Steve: Ok Bill. Your turn.
Bill: alrighty:
Steve: GUI GUI GU- OUCH! That really hurt bill
po GUIMACHINE123 Септембар 16, 2013

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Get Up In , when seeing one of your friends creepin on a person you yell it as a code word as to not let the person whos getting creeped on know that you just told you friend to have sex with that person
at the bar or at school "dam josh is really creepin on that chick, G.U.I. bro!! "
po suga ehans Април 3, 2011

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Gui
A handsome hunk of a man, who is gay
Girl 1: Look it´s Gui
Girl 2: I WANNA TOUCH HIM
Guy: Back off he is MINE!!
po SandHobgoblin Новембар 20, 2014

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The act of drinking a large amount of alcohol, and gobbling like a turkey.
Jim drank a whole bottle of Dack Janiels and gobbled loudly and waddled around like a turkey for well over ten minutes. Then soon after, a police officer tasered him, cuffed him, and took him into custody. He's going to do six months community service for his G.U.I. That poor bastard.
po CHEEZITJEzUS Јул 19, 2013

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